Coding

Part:BBa_K1973018

Designed by: Alvaro Escobar Doncel   Group: iGEM16_UPO-Sevilla   (2016-10-12)


nahR-Psal-nasF-pleD*

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. pleD* (see BBa_K1973016) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal<i> promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BamHI site found at 1188
    Illegal BamHI site found at 2345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 1603
    Illegal NgoMIV site found at 1750
    Illegal AgeI site found at 2086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1261

[edit]
Categories
Parameters
None